The game will kick-off at 7.45pm and will be played a week ahead of the other Worthington Cup matches because of Ipswich's involvment in the UEFA Cup.
Town travel to Yugoslavia for the second-leg of their UEFA tie against Sartid on Thursday, October 3rd.
Worthington Cup draw in full:
Stockport County v Gillingham
Crystal Palace v Cheltenham Town
Leyton Orient v Birmingham City
Cambridge United v Sunderland
Ipswich Town v Brighton
Sheffield United v Wycombe
Wrexham v Everton
Rotherham v Wolves
Chesterfield v West Ham
Charlton v Oxford United
Tottenham v Cardiff City
Aston Villa v Luton Town
Sheffield Wednesday v Leicester City
Wigan v West Brom
Coventry City v Rushden & D
Bolton v Bury
Southampton v Tranmere
Brentford v Middlesbrough
Portsmouth v Wimbledon
Nottm Forest v Walsall
Manchester City v Crewe
Huddersfield v Burnley
Derby v Oldham
Macclesfield v Preston North End
All ties (apart from Ipswich) to be played week commencing Monday, September 30th.
* Eight Premier League teams get byes because of their involvement in Europe and come into the competition in the Third Round, they are: Arsenal, Liverpool, Manchester United, Newcastle United, Chelsea, Leeds United, Blackburn Rovers and Fulham.